![]() 3,4 Given their versatile properties, such polymer capsules have been engineered to be readily internalised by a wide range of cell lines, including epithelial cells and various immune cells. ![]() ![]() 1,2 In particular, nanoengineered polymer capsules fabricated through layer-by-layer (LbL) assembly are promising candidates for the intracellular delivery of therapeutics due to the control that can be exerted over their surface chemistry, size, shape, and cargo loading/release. 1 Introduction Over the past two decades, polymer capsules have evolved from hollow polyelectrolyte constructs to promising tailor-made nanoreactors and drug delivery systems. ![]() Furthermore, this study may aid in the design of novel drug carriers that are sensitive to deformation for enhanced drug release properties. Our study demonstrates the use of super-resolution SIM in analysing intracellular capsule deformation, offering important insights into the cellular processing of drug carriers in cells and providing fundamental knowledge of intracellular mechanobiology. This suggests that the mechanical forces, which induce capsule deformation during cell uptake, vary between cell lines, indicating that the capsules are exposed to higher mechanical forces in HeLa cells, followed by RAW264.7 and then differentiated THP-1 cells. We observed that the deformation of capsules was dependent on cell line, but independent of capsule shape. Spherical- and cylindrical-shaped capsules were studied in three different cell lines, namely HeLa (human epithelial cell line), RAW264.7 (mouse macrophage cell line) and differentiated THP-1 (human monocyte-derived macrophage cell line). Herein we examine the intracellular deformation of layer-by-layer assembled polymer capsules using super-resolution structured illumination microscopy (SIM). Understanding the behaviour of therapeutic carriers is important in elucidating their mechanism of action and how they are processed inside cells.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |